episomal vector (Addgene inc)
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Addgene inc
episomal vector
Episomal Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/px458+aavs1/PX458-AAVS1+(Plasmid+%23113194)/10__34133_slash_research__1241-227-9-12
Average 93 stars, based on 30 article reviews
Episomal Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/px458+aavs1/PX458-AAVS1+(Plasmid+%23113194)/10__34133_slash_research__1241-227-9-12
Average 93 stars, based on 30 article reviews
episomal vector - by Bioz Stars,
2026-09
93/100 stars
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Plasmid Preparation:Article Title: miR126-mediated impaired vascular integrity in Rett syndrome Article Snippet: .. Article Title: miR126-mediated alteration of vascular integrity in Rett syndrome. Article Snippet: .. Article Title: The PST repeat region of MDC1 is a tunable multivalent chromatin tethering domain Article Snippet: To knock-in inducible WT and T>A GFP-PST-LacI fusion proteins into the AAVS1 locus in U2OS 2-6-3 cells, we PCR amplified GFP and LacI from a GFP-LacI expression plasmid (a kind gift from Iain Cheeseman) 68 and codon-optimized WT and T>A PST repeat sequences and cloned them by Gibson assembly into MluI and SalI-linearized AAVS1-TRE3G-EGFP (a gift from Su-Chun Zhang (Addgene plasmid # 52343)) . .. The AAVS1-targeting gRNA plasmid, Over Expression:Article Title: ZMYND11 functions in bimodal regulation of latent genes and brain-like splicing to safeguard corticogenesis. Article Snippet: .. Generation of doxycycline inducible ZMYND11 overexpression lines In experiments where we overexpressed the wildtype ZMYND11 construct, Construct:Article Title: ZMYND11 functions in bimodal regulation of latent genes and brain-like splicing to safeguard corticogenesis. Article Snippet: .. Generation of doxycycline inducible ZMYND11 overexpression lines In experiments where we overexpressed the wildtype ZMYND11 construct, Article Title: ZMYND11 Functions in Bimodal Regulation of Latent Genes and Brain-like Splicing to Safeguard Corticogenesis Article Snippet: .. In experiments where we overexpressed the wildtype ZMYND11 construct, Article Title: ZMYND11 functions in bimodal regulation of latent genes and brain-like splicing to safeguard corticogenesis Article Snippet: .. In experiments where we overexpressed the wildtype ZMYND11 construct, Transfection:Article Title: The Generation of Genetically Engineered Human Induced Pluripotent Stem Cells Overexpressing IFN-β for Future Experimental and Clinically Oriented Studies Article Snippet: On the next day, the cells were dissociated using the TrypLE Express Enzyme (Thermo Fisher Scientific), and counted, and 10 6 cells were transfected using the NeonTM Transfection System 100 μL Kit (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions (NeonTM NxT Electroporation System, InvitrogenTM, Thermo Fisher Scientific, Waltham, MA, USA; one pulse, 30 ms, 1100 V). .. The plasmids used for the transfection were as follows: pAAVS1-hPGK-IFNB1 (expressing the human IFNB ORF) and Expressing:Article Title: The Generation of Genetically Engineered Human Induced Pluripotent Stem Cells Overexpressing IFN-β for Future Experimental and Clinically Oriented Studies Article Snippet: On the next day, the cells were dissociated using the TrypLE Express Enzyme (Thermo Fisher Scientific), and counted, and 10 6 cells were transfected using the NeonTM Transfection System 100 μL Kit (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions (NeonTM NxT Electroporation System, InvitrogenTM, Thermo Fisher Scientific, Waltham, MA, USA; one pulse, 30 ms, 1100 V). .. The plasmids used for the transfection were as follows: pAAVS1-hPGK-IFNB1 (expressing the human IFNB ORF) and |